pd 1 pe (Miltenyi Biotec)
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pd 1 pe
Pd 1 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pd+1+pe/CD274+(B7-H1)+Antibody%2C+anti-human%2C+REAfinity/pmc12969026-254-40-47
Average 94 stars, based on 14 article reviews
Pd 1 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pd+1+pe/CD274+(B7-H1)+Antibody%2C+anti-human%2C+REAfinity/pmc12969026-254-40-47
Average 94 stars, based on 14 article reviews
pd 1 pe - by Bioz Stars,
2026-09
94/100 stars
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1) Product Images from "Antigen-binding affinity is a key determinant of the durable antitumor activity of CD5 CAR-T cells"
Article Title: Antigen-binding affinity is a key determinant of the durable antitumor activity of CD5 CAR-T cells
Journal: Molecular Therapy Oncology
doi: 10.1016/j.omton.2026.201158
Figure Legend Snippet: Low-affinity CD5 CAR-T cells exhibit reduced fratricide and exhaustion phenotypes (A) Viability of CAR-T cells measured on day 3 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (B) Culture supernatants were collected on day 3 after cell seeding. IFN-γ and TNF-α levels secreted by CAR -T cells were measured by cytometric bead array. Data are presented as mean ± SD from multiple donors ( n = 3). (C) CD69 expression level of CAR-T cells on day 10 after seeding. Fluorescence intensity was quantified by flow cytometry. Data are presented as mean ± SD from multiple donors ( n = 3). (D) Total T cell number harvested on day 10 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (E) Expression levels of PD-1 and LAG-3 (surface markers), and TOX (intracellular marker) in CAR-T cells on day 10 after seeding. Surface markers were analyzed by flow cytometry, and TOX expression was assessed via intracellular staining following fixation and permeabilization. Data are presented as mean ± SD from multiple donors ( n = 3). All statistical significance was assessed using a linear mixed-effects model with donor as a random effect and Tukey’s test.
Techniques Used: Expressing, Fluorescence, Flow Cytometry, Marker, Staining
Figure Legend Snippet: Affinity-tuned A2 variants provide functional evidence that antigen-binding affinity plays a key role in regulating fratricide and T cell exhaustion (A) Binding level of recombinant anti-CD5 scFvs to CD5-positive and CD5-negative cell lines, as measured by flow cytometry. Data are presented as mean ± SD from technical replicates ( n = 3). (B) Affinity properties of anti-CD5 scFvs as determined by biolayer interferometry using Octet system. A2 scFv was used as controls. (For definitions of K D , K a , K dis , and curve fit parameters, refer to legend.) (C) Viability of CAR-T cells measured on day 3 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (D) Culture supernatants were collected on day 3 to assess IFN-γ and TNF-α secretion, measured using cytometric bead array. Data are presented as mean ± SD from technical replicates ( n = 3). (E) Total T cell number harvested on day 10 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (F) CD69 expression levels in CAR-T cells on day 10 after cell seeding, measured by surface staining and flow cytometry. Data are presented as mean ± SD from multiple donors ( n = 3). (G) Expression levels of PD-1 and LAG-3 (surface markers) and TOX (intracellular) in CAR-T cells on day 10 after seeding. Surface markers were analyzed by flow cytometry, and TOX expression was assessed via intracellular staining. Data are presented as mean ± SD from multiple donors ( n = 3). (H) 3 × 10 5 Myc + CAR-T cells were co-cultured with 1 × 10 5 Jurkat cells every 3–4 days for repeated antigen stimulation. Expansion of Myc + CAR-T cells was measured weekly by flow cytometry. Data are presented as mean ± SD from technical replicates ( n = 3). Statistical analyses were performed using one-way ANOVA with Tukey’s multiple comparisons test for (A, D, and H) and a linear mixed-effects model with donor as a random effect followed by Tukey’s test for (C–G).
Techniques Used: Functional Assay, Binding Assay, Recombinant, Flow Cytometry, Expressing, Staining, Cell Culture
Figure Legend Snippet: High-affinity C7 variant-based CAR5 exhibited increased levels of both fratricide and T cell exhaustion compared to the C7 wild-type CAR5 (A) Binding level recombinant anti-CD5 scFvs to CD5-positive and CD5-negative cell lines, as measured by flow cytometry. Data are presented as pooled mean ± SD from technical replicates ( n = 3). (B) Affinity properties of anti-CD5 scFvs as determined by biolayer interferometry using Octet system. C7 scFv was used as controls. (For definitions of K D , K a , K dis , and curve fit parameters, refer to legend.) (C) Viability of CAR-T cells measured on day 3 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (D) Culture supernatants were collected on day 3 to assess IFN-γ and TNF-α secretion, measured using cytometric bead array. Data are presented as mean ± SD from technical replicates ( n = 3). (E) Total T cell number harvested on day 10 after cell seeding. Data are presented as mean ± SD from multiple donors ( n = 3). (F) CD69 expression levels in CAR-T cells on day 10 after cell seeding, measured by surface staining and flow cytometry. Data are presented as mean ± SD from multiple donors ( n = 3). (G) Expression levels of PD-1 and LAG-3 (surface markers) and TOX (intracellular) in CAR-T cells on day 10 after seeding. Surface markers were analyzed by flow cytometry, and TOX expression was assessed via intracellular staining. Data are presented as mean ± SD from multiple donors ( n = 3). (H) 3 × 10 5 Myc + CAR-T cells were co-cultured with 1 × 10 5 Jurkat cells every 3–4 days for repeated antigen stimulation. Expansion of Myc + CAR-T cells was measured weekly by flow cytometry. Data are presented as mean ± SD from technical replicates ( n = 3). Statistical analyses were performed using one-way ANOVA with Tukey’s multiple comparisons test for (A, D, and H) and a linear mixed-effects model with donor as a random effect followed by Tukey’s test for (C–G).
Techniques Used: Variant Assay, Binding Assay, Recombinant, Flow Cytometry, Expressing, Staining, Cell Culture
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To verify PD-1 expression, NK cells were stained with CD56-Pe-Cy7 and Article Title: GD2-targeted chimeric antigen receptor T cells prevent metastasis formation by elimination of breast cancer stem-like cells Article Snippet: On day +7, CAR transduction efficiency and CD4/CD8 ratio were determined by flow cytometry using the antibodies: CD4-BUV395 (SK3, BD Bioscience), CD8-APC (BW135/80, Miltenyi Biotec) and EGFR-PE (AY13, BioLegend). .. Tonic signaling was assessed by expression of the exhaustion markers PD-1, TIM-3, and LAG-3 via flow cytometry on day +21 of culturing using the antibodies: EGFR-FITC (13/EGFR (RUO), BD Bioscience); Flow Cytometry:Article Title: GD2-targeted chimeric antigen receptor T cells prevent metastasis formation by elimination of breast cancer stem-like cells Article Snippet: On day +7, CAR transduction efficiency and CD4/CD8 ratio were determined by flow cytometry using the antibodies: CD4-BUV395 (SK3, BD Bioscience), CD8-APC (BW135/80, Miltenyi Biotec) and EGFR-PE (AY13, BioLegend). .. Tonic signaling was assessed by expression of the exhaustion markers PD-1, TIM-3, and LAG-3 via flow cytometry on day +21 of culturing using the antibodies: EGFR-FITC (13/EGFR (RUO), BD Bioscience); Article Title: Analysis of the mechanisms regulating soluble PD-1 production and function in human NK cells. Article Snippet: For PD-1 induction, NK cells were stimulated for six days with a cocktail of cytokines consisting of IL-12 (10 ng/ml), IL-15 (25 ng/ml), IL-18 (100 ng/ml) (all purchased from Myltenyi) and DMSO (1:2000; SIGMA) (control NK cells) or 1mMDexamethasone (Stimulated NK cells) (1:2000, SIGMA) (stimulated NK cells). .. To verify PD-1 expression, NK cells were stained with CD56-Pe-Cy7 and Article Title: Analysis of the mechanisms regulating soluble PD-1 production and function in human NK cells Article Snippet: For PD-1 induction, NK cells were stimulated for six days with a cocktail of cytokines consisting of IL-12 (10 ng/ml), IL-15 (25 ng/ml), IL-18 (100 ng/ml) (all purchased from Myltenyi) and DMSO (1:2000; SIGMA) (control NK cells) or 1mM Dexamethasone (Stimulated NK cells) (1:2000, SIGMA) (stimulated NK cells). .. To verify PD-1 expression, NK cells were stained with CD56-Pe-Cy7 and Article Title: GD2-targeted chimeric antigen receptor T cells prevent metastasis formation by elimination of breast cancer stem-like cells Article Snippet: On day +7, CAR transduction efficiency and CD4/CD8 ratio were determined by flow cytometry using the antibodies: CD4-BUV395 (SK3, BD Bioscience), CD8-APC (BW135/80, Miltenyi Biotec) and EGFR-PE (AY13, BioLegend). .. Tonic signaling was assessed by expression of the exhaustion markers PD-1, TIM-3, and LAG-3 via flow cytometry on day +21 of culturing using the antibodies: EGFR-FITC (13/EGFR (RUO), BD Bioscience); Staining:Article Title: GD2-targeted chimeric antigen receptor T cells prevent metastasis formation by elimination of breast cancer stem-like cells Article Snippet: On day +7, CAR transduction efficiency and CD4/CD8 ratio were determined by flow cytometry using the antibodies: CD4-BUV395 (SK3, BD Bioscience), CD8-APC (BW135/80, Miltenyi Biotec) and EGFR-PE (AY13, BioLegend). .. Tonic signaling was assessed by expression of the exhaustion markers PD-1, TIM-3, and LAG-3 via flow cytometry on day +21 of culturing using the antibodies: EGFR-FITC (13/EGFR (RUO), BD Bioscience); Article Title: Analysis of the mechanisms regulating soluble PD-1 production and function in human NK cells. Article Snippet: For PD-1 induction, NK cells were stimulated for six days with a cocktail of cytokines consisting of IL-12 (10 ng/ml), IL-15 (25 ng/ml), IL-18 (100 ng/ml) (all purchased from Myltenyi) and DMSO (1:2000; SIGMA) (control NK cells) or 1mMDexamethasone (Stimulated NK cells) (1:2000, SIGMA) (stimulated NK cells). .. To verify PD-1 expression, NK cells were stained with CD56-Pe-Cy7 and Article Title: Analysis of the mechanisms regulating soluble PD-1 production and function in human NK cells Article Snippet: For PD-1 induction, NK cells were stimulated for six days with a cocktail of cytokines consisting of IL-12 (10 ng/ml), IL-15 (25 ng/ml), IL-18 (100 ng/ml) (all purchased from Myltenyi) and DMSO (1:2000; SIGMA) (control NK cells) or 1mM Dexamethasone (Stimulated NK cells) (1:2000, SIGMA) (stimulated NK cells). .. To verify PD-1 expression, NK cells were stained with CD56-Pe-Cy7 and Article Title: GD2-targeted chimeric antigen receptor T cells prevent metastasis formation by elimination of breast cancer stem-like cells Article Snippet: On day +7, CAR transduction efficiency and CD4/CD8 ratio were determined by flow cytometry using the antibodies: CD4-BUV395 (SK3, BD Bioscience), CD8-APC (BW135/80, Miltenyi Biotec) and EGFR-PE (AY13, BioLegend). .. Tonic signaling was assessed by expression of the exhaustion markers PD-1, TIM-3, and LAG-3 via flow cytometry on day +21 of culturing using the antibodies: EGFR-FITC (13/EGFR (RUO), BD Bioscience); Software:Article Title: Analysis of the mechanisms regulating soluble PD-1 production and function in human NK cells. 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To verify PD-1 expression, NK cells were stained with CD56-Pe-Cy7 and Recombinase Polymerase Amplification:Article Title: Antigen-binding affinity is a key determinant of the durable antitumor activity of CD5 CAR-T cells Article Snippet: For detection of cell surface-bound anti-CD5 recombinant scFv, 2 × 10 5 cells were incubated with 10 ng of mIgG2a-Fc-tagged anti-CD5 scFv protein (Y-Biologics, Republic of Korea), washed, and subsequently stained with AF647-conjugated anti-mIgG2a antibody (clone RMG2a-62, BioLegend). .. The following antibodies were used: BioLegend, CD5-PE (clone UCHT2), CD69-PE (clone FN50), LAG3-FITC (Clone 7H2C65), mIgG2a-AF647 (clone RMG2a-62), and streptavidin-AF647; BD Biosciences, CD4-V500 (clone RPA-T4), CD8-V450 (clone RPA-T8), αβ TCR-PE (clone IP26), and γδ TCR-BV421 (clone 11F2); Thermo Fisher Scientific, Article Title: Antigen-binding affinity is a key determinant of the durable antitumor activity of CD5 CAR-T cells Article Snippet: For detection of cell surface-bound anti-CD5 recombinant 430 scFv, 2 x 105 cells were incubated with 10 ng of mIgG2a-Fc-tagged anti-CD5 scFv protein 431 (Ybiologics, Republic of Korea), washed, and subsequently stained with AF647-conjugated 432 anti-mIgG2a antibody (clone RMG2a-62, BioLegend). .. The following antibodies were used: 433 BioLegend, CD5-PE (clone UCHT2), CD69-PE (clone FN50), LAG3-FITC (Clone 7H2C65), 434 mIgG2a-AF647 (clone RMG2a-62) and Streptavidin-AF647; BD Biosciences, CD4-V500 435 (clone RPA-T4), CD8-V450 (clone RPA-T8), αβ TCR-PE (clone IP26), and γδ TCR-BV421 436 (clone 11F2); Thermo Fisher Scientific, |
